An improved electron microscopic technique for the immunolabeling of Cryptosporidium parvum oocysts.
نویسندگان
چکیده
A technique was developed for immunolabeling Cryptosporidium parvum oocysts for subsequent observation by transmission electron microscopy. This method was developed to maintain architectural integrity of the oocyst wall and improve fixation of internal contents. The improved fixation and embedding method permits efficient immunolabeling of both nonexcysted and excysted C. parvum oocysts and may be applicable to other oocyst- and cyst-forming protozoa.
منابع مشابه
Detection of Cryptosporidium parvum oocysts in calf fecal samples by direct immunofluorescence assay Detecção de oocistos de Cryptosporidium parvum em amostras fecais de bezerros pela reação de imunofluorescência direta
The aim of this study was to produce a conjugate containing anti-Cryptosporidium parvum polyclonal antibodies and standardize a Direct Immunofluorescence Assay (DIF) for detecting C. parvum oocysts in fecal samples from calves. In order to obtain anti-C. parvum polyclonal antibodies, two New Zealand rabbits were immunized with a purified solution of C. parvum oocysts and Freund’s adjuvant. Puri...
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ورودعنوان ژورنال:
- The Journal of parasitology
دوره 92 2 شماره
صفحات -
تاریخ انتشار 2006